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American Society of Microbiology ABMM Sample Questions:

1. A diagnostic laboratory is validating a new multiplex PCR panel for respiratory pathogens. According to CLIA regulations, which of the following parameters must be established by the laboratory performing the validation for this qualitative nucleic acid amplification test?

A) Accuracy, Precision, Reportable Range, Analytical Sensitivity
B) Accuracy, Precision, Clinical Sensitivity, Clinical Specificity
C) Precision, Reportable Range, Analytical Specificity, Reference Interval
D) Accuracy, Precision, Analytical Sensitivity, Analytical Specificity (including cross-reactivity)

2. A patient develops a febrile illness with a diffuse petechial rash after a camping trip. Serological testing reveals a four-fold rise in antibody titer to Rickettsia rickettsii. The vector responsible for transmitting this organism is a:

A) Mosquito
B) Tick
C) Flea
D) Louse

3. A patient with HIV infection and a CD4+ T-cell count below 50 cells/µL develops a progressive neurological illness characterized by confusion, motor deficits, and seizures. Brain MRI reveals multiple white matter lesions without mass effect. PCR of the CSF is positive for JC virus. Thepathogenesis of this condition, progressive multifocal leukoencephalopathy (PML), involves:

A) Lytic infection of oligodendrocytes by JC virus, leading to demyelination.
B) Direct cytopathic effect of JC virus on neurons.
C) Inflammatory demyelination triggered by JC virus infection of astrocytes.
D) Opportunistic infection by JC virus due to impaired cell-mediated immunity.

4. A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?

A) Clostridium perfringens
B) Fusobacterium nucleatum
C) Prevotella melaninogenica group
D) Bacteroides fragilis group

5. A research laboratory is investigating the mechanisms of antigenic variation in Trypanosoma brucei, the causative agent of African trypanosomiasis. The parasite evades the host immune response by periodically changing its surface coat protein. This process primarily involves:

A) Recombination between different VSG gene loci on minichromosomes.
B) Segmental gene conversion events involving a large repertoire of silent VSG genes.
C) RNA interference-mediated silencing of expressed VSG genes followed by activation of a new VSG gene.
D) High-frequency point mutations in the gene encoding the variant surface glycoprotein (VSG).

Solutions: