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American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is studying the pathogenesis of respiratory syncytial virus (RSV) infection in infants.
They find that a specific viral surface glycoprotein is primarily responsible for attachment to host cells. This glycoprotein is:

A) Hemagglutinin (H)
B) Fusion protein (F)
C) Matrix protein (M)
D) Neuraminidase (N)

2. During a primary infection with Epstein-Barr virus (EBV), the virus establishes latency in B lymphocytes.
The transition from lytic to latent infection is tightly regulated by viral gene expression. Which of the following viral proteins is crucial for the establishment and maintenance of EBV latency and is a key target in EBV-associated lymphomas?

A) Glycoprotein gp350
B) Early antigen (EA)
C) Viral capsid antigen (VCA)
D) Epstein-Barr nuclear antigen 1 (EBNA1)

3. A clinical microbiology laboratory is performing Gram staining on a sputum sample. After adding the crystal violet stain, a delay in the washing step with decolorizer (alcohol-acetone) would MOST likely result in:

A) Gram-negative bacteria appearing purple.
B) Gram-positive bacteria appearing pink.
C) Poor adherence of the stain to Gram-positive bacteria.
D) Increased background staining.

4. A patient presents with symptoms suggestive of primary syphilis. Which diagnostic test typically becomes positive earliest in the course of infection?

A) Darkfield microscopy of lesion exudate
B) Treponema pallidum particle agglutination (TP-PA)
C) Rapid Plasma Reagin (RPR)
D) Venereal Disease Research Laboratory (VDRL) test on CSF

5. A patient with a history of neutropenia following chemotherapy develops a rapidly progressive pneumonia.
Bronchoalveolar lavage fluid reveals septate hyphae with acute angle (45-degree) branching. The MOST likely diagnosis is invasive pulmonary aspergillosis caused by:

A) Rhizopus species
B) Fusarium species
C) Aspergillus fumigatus
D) Mucor species

Solutions: