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American Society of Microbiology ABMM Sample Questions:

1. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Multilocus sequence typing (MLST)
B) PCR Ribotyping
C) Pulsed-field gel electrophoresis (PFGE)
D) Whole-genome sequencing (WGS) SNP analysis

2. A traveler returning from sub-Saharan Africa presents with cyclical fevers, chills, and sweats. A Giemsa- stained peripheral blood smear reveals multiple ring forms within red blood cells, some applique forms, and occasional banana-shaped gametocytes. No Schüffner's dots or red blood cell enlargement is observed. Which Plasmodium species is the most likely cause?

A) Plasmodium vivax
B) Plasmodium ovale
C) Plasmodium malariae
D) Plasmodium falciparum

3. A patient with a history of travel to a rural area develops a febrile illness with myalgias and thrombocytopenia. Laboratory testing reveals the presence of morulae within circulating leukocytes. The MOST likely diagnosis is:

A) Ehrlichiosis
B) Babesiosis
C) Anaplasmosis
D) Lyme disease

4. A research laboratory is investigating the quorum sensing system in Pseudomonas aeruginosa. They identify a novel autoinducer molecule and a corresponding transcriptional regulator. Disruption of the gene encoding this regulator leads to a significant decrease in the production of virulence factors such as elastase and pyocyanin. Further analysis reveals that this regulator directly binds to the promoter regions of these virulence genes in the presence of the autoinducer. This regulatory mechanism is an example of:

A) A global regulatory network controlled by alternative sigma factors.
B) A two-component regulatory system involving a sensor kinase and a response regulator.
C) A post-transcriptional regulatory mechanism involving small regulatory RNAs.
D) A direct transcriptional activation mechanism dependent on a small signaling molecule.

5. A clinical laboratory performs a cefoxitin disk diffusion test on a Staphylococcus aureus isolate from a wound culture. The zone of inhibition measures 18 mm. According to current CLSI M100 guidelines, how should this result be interpreted regarding oxacillin/methicillin susceptibility?

A) Susceptible (report oxacillin as susceptible)
B) Intermediate (requires MIC testing)
C) Indeterminate (requires mecA gene testing)
D) Resistant (report oxacillin as resistant; infer MRSA)

Solutions: