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American Society of Microbiology ABMM Sample Questions:

1. A microbiology laboratory is processing a throat swab for the detection of Group A Streptococcus. Culture on sheep blood agar reveals beta-hemolytic colonies. Which of the following tests can be used to presumptively identify Streptococcus pyogenes?

A) Catalase test
B) Bile esculin hydrolysis
C) Coagulase test
D) Bacitracin susceptibility

2. A patient with a history of valvular heart disease undergoes a dental procedure and subsequently develops a bloodstream infection with Streptococcus mutans. This organism gains access to the bloodstream primarily due to:

A) Formation of a protective biofilm on the teeth.
B) Transient bacteremia resulting from the procedure.
C) Production of potent extracellular enzymes.
D) Active invasion of the oral mucosa.

3. A clinical microbiology laboratory is implementing a new laboratory information system (LIS). A critical step in the validation process is ensuring the accurate and reliable transfer of data, including patient demographics, test orders, and results. This aspect of validation primarily focuses on:

A) Clinical sensitivity and specificity.
B) Analytical sensitivity and specificity.
C) Data integrity and interoperability.
D) Workflow efficiency and user training.

4. A patient with cystic fibrosis experiences a pulmonary exacerbation. Sputum culture grows a mucoid strain of Pseudomonas aeruginosa. The mucoid phenotype is primarily due to the overproduction of:

A) Alginate
B) Pyocyanin
C) Exotoxin A
D) Elastase

5. A patient with a history of diabetes mellitus develops a severe foot ulcer. Deep tissue cultures grow multiple anaerobic bacteria, including Clostridium perfringens. The MOST concerning virulence factor produced by this organism in this context is:

A) Botulinum toxin
B) Hyaluronidase
C) Alpha-toxin (lecithinase)
D) Tetanospasmin

Solutions: