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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Monoclonal antibodies are monospecific antibodies that are the same because they are made by one type of immune cell which are all clones of a unique parent cell, also called a hybrid cell line, which usually arise from a hybridoma. The fusion of a specific antibody-producing lymphocyte with a myeloma cell will multiply to become a source of pure monoclonal antibody. This is often used in the manufacturing process for monoclonal antibody reagents.
Monoclonal antibodies are usually manufactured in vitro by using:
A) Hybridomas
B) Human plasma cells
C) Cultured T cells
D) Cytotoxic T cell
2. Each of the drugs/drug classes listed above have been known to cause drug-induced hemolytic anemia, although cephalosporins are the MOST COMMON cause. Cephalosporins can cause drug-induced hemolytic anemia when a patient produces antibodies to the particular cephalosporin drug in the presence of red blood cells. The drugs can alter the membrane appearance of the red blood cells, causing the body to mistake them as foreign. Complement becomes activated due to these antibodies; red cells are then destroyed causing hemolytic anemia. Dark urine, caused by intravascular hemolysis, is one of the most common symptoms associated with this condition.
Which one of the following drugs/drug classes is the MOST COMMON cause of drug-induced hemolytic anemia?
A) Levofloxacin
B) Levodopa
C) Quinidine
D) Cephalosporins
E) Nonsteroidal anti-inflammatory drugs (NSAIDs)
3. The molecular weight of a hapten is by itself not sufficient to elicit an antibody response alone, but requires a protein to do so.
A hapten is only antigenic when it is coupled with which of the following:
A) Enzymes
B) Lymphocytes
C) Proteins
D) Lipids
4. Considering the reactions given in this case study, Yersinia enterocolitica would be the best choice.
Most strains of Plesiomonas shigelloides, Escherichia coli, and Pasteurella multocida are urease negative.
Also, Plesiomonas shigelloides demonstrates a K/A reaction on a TSI slant.
The stool specimen from a patient admitted to the hospital with symptoms of appendicitis has a culture with the following characteristics:
Gram-negative bacillus
Catalase positive
Urease positive
Weakly fermentative
TSI Slant is A/A
These reactions suggest that the organism is MOST likely:
A) Yersinia enterocolitica
B) Escherichia coli
C) Plesiomonas shigelloides
D) Pasteurella multocida
5. Francisella is slow growing on primary isolation culture media and produces poorly-staining coccobacilli on Gram stain. Because Francisella tularensis is included on the list of bioterrorism agents, suspicious isolates should be referred immediately to a public health laboratory in lieu of attempting an in-laboratory identification.
One of the BIGGEST problems with isolating Francisella is that these organisms:
A) grow so slowly
B) produce greenish colonies like E. coli
C) overgrow other organisms
D) produce wide zones of hemolysis
Solutions:
Question # 1 Answer: A | Question # 2 Answer: D | Question # 3 Answer: C | Question # 4 Answer: A | Question # 5 Answer: A |